This article discusses the general experimentalĬonsiderations and design for live plant imaging studies. Therefore, optimized culturingĪnd imaging conditions are essential for successful live cell studies in plants. (having multiple cell layers), and can have strong autofluorescence, especially in green leaves. Moreover, plant tissues are normally thick In addition, plantsĪlso have strong circadian rhythms of physiological behavior and gene expression. Must keep in mind that plants sense light, temperature, osmolarity, humidity, gravity, and nutrition. In designing live imaging experiments, one While plant growth and development are inherently multiscale, conventional light, fluorescence, and electron microscopy platforms are typically limited to imaging of plant microstructure from small flat samples that lack direct spatial context. Investigation of the molecular mechanisms underlying various cellular phenomena. Capturing complete internal anatomies of plant organs and tissues within their relevant morphological context remains a key challenge in plant science. In addition, the availability of powerful genetic tools facilitates the Hyperspectral imaging microscopy may result in new spatial information on structural and chemical modifications of the plant tissue in the course of host-pathogen interactions. Nucleus contains only five pairs of chromosomes in which heterochromatin domains are organized as chromocenters, and theseĭomains are easily resolved under the microscope. Near-range imaging sensor systems and spectroradiometry may be also used in plant pathology for investigating the effect of pathogenesis on the cellular level. The tissues, and its plantlets contain different cell types with different ploidy levels and differentiation stages. Its small size allows fluorescent light to penetrate The tiny flowering plant, Arabidopsis thaliana, provides an ideal system to apply various live microscopy techniques.

Under normal or stressed growth conditions. Live cell imaging is an essential approach for studying the structure, dynamics, and functions of cells in a living plant

Plant imaging